Micromanipulation systems allow non-contact trapping of biological molecules such as cells and DNA by harnessing the radiation pressure of a laser beam. The trapped sample moves freely in the microscopic field. Using a near-infrared laser (fÉ=1,064 nm), which is less harmful to biological components, the system allows independent manipulation of two beams by the high-efficiency laser-branching method, enabling applications involving not just the trapping of a single particle, but also studies of the tensile strength of fibrous tissues and intercellular interactions. Micromanipulation systems with a single trapping laser beam are also available.

Both systems enable trapping together with fluorescence observation or the like. Custom microscopes (e.g. with integrated laser systems) are also available. Please contact us for more information.

Key features of Sigma Koki products

BE: Beam expander
WP: Wave plate
ND1 and ND2: ND filters
L1 and L2: Lenses
OL: Objective lens
M1 to M3: Dielectric mirrors
PBS1 and PBS2: Polarization beam splitters
DM: Dichroic mirror

In optical trapping with near-infrared lasers, two manipulation beams are used to enable more complex operations than a single fixed beam would allow.

Sigma Koki employs a two-beam method based on a polarization beam splitter, based on Sigma Koki's proprietary optical design. Each beam can be moved freely in the microscopic field by adjusting elements of the optical system. The optical trapping technology demands construction of a light source of superior quality and an optical system with minimal light loss.